Select one skill from the Year 1 Essential Skill Clusters that you Essay

Select one skill from the Year 1 Essential Skill Clusters that you achieved in your first practice placement and had signed by y - Essay Example She also gave me pointers on how I could improve in my next placement. The mentor also provided feedback from the patients, and through such feedback, I found out that the patients liked me talking to them and smiling at them. In general therefore, I performed well. Part 1: Gibbs Reflective cycle Description (What happened?) The incident involved my clinical placement with a community hospital which houses 19 inpatient beds, an A & E Department, Physiotherapy, and X-Ray/Ultrasound facilities. Their services include rehabilitation for patients having gone through accidents or minor injuries with a team of professionals including doctors, nurses, physiotherapists, occupational therapists, and social workers/case managers. During this placement, I was able to apply various skills and take part in various nursing interventions. One of the activities I was able to participate in is the handover during multidisciplinary team meetings. The meeting comprised of various nurses and other healt h professionals caring for different patients. To protect patients’ confidentiality in accordance with the NMC Code of Conduct, patients’ consent for care were sought before any essential nursing services were offered (NMC Code of Conduct Domain 2.8). I administered care to patients in the ward, assisting in wound cleaning and vital signs monitoring. The handover multidisciplinary meetings were done weekly with nurses bringing all the folders for the patients in the bay and giving a handover to everyone attending the meeting. I participated in giving handovers to about two patients a week. Members of the team ask questions about the patients I would be handing over, and I would explain what I can about each patient. At times, my mentor would help me explain further about each patient. The patients’ attending physicians, physiotherapists, occupational therapists, nurses, dieticians, and social workers were present in the meeting. I reported about the patientsâ₠¬â„¢ vital signs, oftentimes indicating possible changes and unstable levels for patients. I also expressed that some patients had a low threshold for pain. The Gibbs reflection style was used for this paper because it provides an orderly and detailed process of reflection. It also allows for an in-depth assessment of details and interventions used during the incident being evaluated. Feelings (What I was thinking and feeling) I felt like my contribution to the team was an important contribution and I was also assured with the fact that the other members of the team listened and welcomed my comments and contribution to the handover meetings. I also listened to them as they shared their expertise on patient care. At times however, I found the discussion intimidating because the members of the team are experts in their field. I felt however, that I needed to be more assertive during these discussions in order to ensure that my contributions to the discussion would be heard. Evaluation (What was good and bad about the incident) What was good about the incident was that it taught me that the contributions of the team in the care of a patient are effective means by which improved patient outcomes can be gained. By attending the handover multidisciplinary meetings, I was able to endorse the patient and communicate their essential needs based on my assessment. With the assistance of my mentor, I was also able to be effective during the handover meetings. What was bad about the experience was that there were

Incident for Aids Case Study Example | Topics and Well Written Essays - 500 words

Incident for Aids - Case Study Example However, the scope ought to be widened to include other men from other races and social classes. The level of diversity among the minority groups such as the Hispanics presented a major challenge for the agency, as it could not replicate the initiatives that had been successful with the original group. This was a dire phenomenon because the epidemic was rising among gay and bisexual males from these minority groups. For instance, the level of acculturation to the American ways has a huge impact on how a man would face the stigma associated with AIDS and homosexuality. Therefore, there was need to develop different sensitive programs that would target the different groups such as the youth, recent migrants and the acculturated. Consensus building entails seeking the consent of all participants in a situation where there were differences in opinion. In formulating a Latino-oriented program, the agency consulted an expert who proposed six barriers for adoption of safer behaviour. The barriers were reduced to four after lengthy discussions, but the agency representatives still hold different opinions about the number that should be used. In order to develop consensus I would appeal to the representatives to focus on the common interest and not their agencies’ position. In this case, the common interest will be identification of the major possible barriers. The focus group must incorporate members from all the segments of the target group. Since there are many members, it is imperative that the stakeholders command respect from the other members, who must exhibit their confidence in them. It is important to select people who have a similar perception of the issues and not those who will undermine each other. A focus group should work towards creating a lasting solution for the issues. Therefore, they should provide this information: what they have learnt from other segments; what each group should do to achieve the goals; and

Management Assignment(LAW) Essay Example | Topics and Well Written Essays - 1000 words

Management Assignment(LAW) - Essay Example Charles Fried stated that persons entering into a contract are morally bound to keep it.*4 Therefore, a contract is equivalent to a written promise to deliver a service or goods in exchange for some consideration and fairness in transactions is the goal sought to be achieved in contract law. Traditional notions of laissez faire have been eschewed in modern contract law. In interpreting contracts and determining their validity, the courts have demonstrated that it is not the meaning a person actually intended to convey but what a reasonable person would have construed it as, which is referred to as the process of â€Å"objective interpretation.*5 In order that a contract be deemed valid, the parties to the contract must be clearly specified and the terms upon which the parties agree to conduct business must also be clearly laid out. In the case of Intel’s suppliers, the contract would therefore exist between Intel and the suppliers, since the engineers are in effect, acting on behalf of Intel and not as independent agents. This would however qualify as a bilateral contract since it involves the two parties: Intel via its engineers and the suppliers. In purchasing their supplies, the engineers functioning as Intel’s agents will be deemed to have made an offer for purchase of goods*6, which will not however be enforceable unless the suppliers have agreed and indicated their agreement through a written contract. An offer which is not communicated to the offerree will be deemed to be invalid or ineffective.*7 Therefore, assuming that Intel’s engineers have communicated their offer to the suppliers and there is a counter proposal made by the supplier in regard to the purchase price of the goods, this will be deemed to negate the original offer.*8 It is then up to the offerer to either agree with the counter proposal and indicate

Monopolistic competition Research Paper Example | Topics and Well Written Essays - 1500 words

Monopolistic competition - Research Paper Example Since then, availability has increased across the world and the industry has grown with emergency and growth of new mobile phone companies because demand for cell phones has gone up. Currently, the smart phone market is the most sought after with players in the industry striving for a place in the market. Among the global leaders in the industry are Samsung, Apple, LG, Nokia, LG, and ZTE, while other minor companies tend to dominate their respective regions of operation. Samsung still leads in the smart phone market (Table 1 and Table 2). This paper discusses Samsung as a mobile manufacturer, and its relation to other players in the industry. As a manufacturing company in a highly competitive industry that thrives on innovation and technology, Samsung has perfected the art of customization in its efforts to differentiate its products from those of its competition. In a bid to avoid commoditization therefore, the company customised their production as much as they could. More than 50 percent of the company’s memory chips have been special orders for other manufacturers including Nokia, Microsoft and Dell. At one instance the firm undertook an effort that was aimed at converting its product line to a high end premium goods one from a previously low end commodity product line. In addition, the company has strived to lay an emphasis on hardware technology as opposed to its competition such as Apple and Samsung who concentrate on getting into proprietary content and software such as movies, music, and games. Though there are many mobile phones, each brand or company exhibits a high level of differentiation in the minds of consumers. This could be as a result of continuous usage and thorough advertising. Therefore, existence of a large number of sellers who sell close substitute products that are differentiated has led to monopolistic competition in the mobile phone industry. Samsung competes with other mobile phone companies by exercising

Body Image Essay Example for Free

Body Image Essay Ones body is what makes one who one is. Every inch of someone is what sets a person apart from everyone else. Even though everyone has an opinion about what his or her perfect body would be, changing ones personal features would take away from individuality. I personally would never have plastic surgery because when I look at myself, I do not see a young woman with a funny looking nose and big bug eyes, but I see a beautifully sculpted masterpiece that God has made just for me. It is a body that gives me character, individuality, and confidence. In my personal opinion, and I say this as humbly as I can, I am a beautiful young lady. I have a very animated face that goes well with my personality, which is humorous, but in a mature fashion. I can be serious and stare at others with my endless snowflake hazel eyes, but if I get too drastic for the mood, it is easy to look at my nose and then laugh to lighten the air. I take pride in the fact that there is no one else on this earth exactly like me. Not just my inner beauty, but my outer beauty makes me different from everyone else. Life would be so boring if everyone looked exactly the same, but what is inside oneself makes one beautiful, not only inside, but outside as well. I personally could not be more content and happy with who I am. If I were to walk down the street, there would be many different comments going through peoples heads as I passed. Some people would find me attractive, and some would find me ugly. Some people might find me beautiful, and others might find me weird looking. The bottom line is that it does not matter what they think. It only matters what I think because I am the only one in the whole world that has to live with myself. I am proud to be me, and no surgery could make me any more confident, or any happier to be me. In conclusion, unless I had a huge rock fall on my face, which made it difficult to eat or breathe properly. I would never have plastic surgery done to any part of my already existing body. I would never second-guess my own character, individuality, or confidence. I am perfect just as God made me.

Analysis of UK and Pakistan Education System Essay Example for Free

Analysis of UK and Pakistan Education System Essay UK is one of the renowned trading power and financial hub of the world, stands at third position in European Union following Germany and France. The agriculture sector of the region is particularly strong and mechanized. Its efficiency can be ensured through the production of 60 percent food needs with only 2 percent of workforce. The region has significant energy resource but due to increasing demands it is importing energy from 2005. The insurance and financial institutes in addition to bank are generating the largest portion of GDP of the region. The recent financial crises particularly influenced the economy of the region during 2008 because of the financial sector of the region (www. cia. gov). Literacy rate of the region is 99 percent while 5. 5 percent of GDP is fixed for the development of education sector. According to such percentage of GDP it stands at 44th position as compare to other countries (www. cia. gov). There were 89 universities and 131 higher education institutes operating till August 2011 (www. universitiesuk. ac. uk). The expansion in higher education system of the region is relatively slow as compared to the other countries competing with the region according to the report of Organisation for Economic Cooperation and Development (OECD, 2007). There are several countries coming up with significantly higher number of college and universities although the growth of education sector in recent years in UK is also remarkable. The increase in the educational institutes positively influence the graduation rate in UK as it was 37 percent during the year 2000 and reached upto 39 percent in 2005. On the other hand the position of the region was dropped from 3rd to 10th position as compare to around 20 countries while considering the educational performance (www. guardian. co. uk). The recent financial crises negatively influenced all the sectors therefore the government was planning to reduce the educational expenses by 25 percent during the upcoming four years. It was mentioned the chancellor that the reduction in the budget for educational sector depends on the treatment with the other sectors which are influencing the budget of the region. No guarantee of not cutting the educational budget was given by the chancellor. It was further mentioned by the government official that the possible saving through reduction in welfare budget may positively facilitate in reducing percentage of cut in educational budget (BBC, 2010). UK is also famous as an esteem education provided all around the world and students of different continents are presently completing their education in UK. Recently the government particularly focus the immigration process of students for making amendments. The seniors of UK education sector that the shift of government towards Tier 4 student immigration program may adversely effect the higher education institutes and the whole economy. The government particularly deployed for deciding about the student visa system of UK according to the new rules and policies. In addition the numbers of immigration given to the skilled workers also threatened the education sector because the student which will not take higher education may reduce the scope of universities and it will be quite difficult for the region to compete with the other countries as far as the educational sector is concerned (www. orkpermit. com). Pakistan is facing severe law and order situation in addition to political disputes which negatively influencing the trend of foreign investment. The recent energy crises are also one of the factors which badly effecting the overall development of economy. The unemployment rate according to the government is 6 percent but the real rate is very high because of the slow economic development. During the recent years the growth of the country significantly reduced while the inflation rate is particularly increasing. On the other hand food prices have also remarkable increased. The UN Human Development Report estimated the poverty during 2011 around 50 percent population. The inflation rate was recorded at 7. 7 percent during 2007 which was crossed 13 percent during the year 2011. The currency value of Pakistan has decreased by 40 percent since 2007 due to such circumstances (www. cia. gov). The education facilities in Pakistan are although developing but still require significant attention to compete with the global market. The literacy rate of the country is very low and estimated as around 50 percent in which 63 percent are males while remaining are females. The government declared 2. 7 percent of annual GDP for the education sector so that further schools can be developed and established according to the need of population. Keeping in view the expenses declared for education sector as compared to the remaining world the country is at 142nd position which is considerably low rank (www. cia. gov). Ministry for education is performing critical role for the development education sector in Pakistan. The key aim is to promote education according to the policies and plans defined by the management of the firm. Under this ministry education policy for 1998-2010 was prepared for significantly focusing the overall development educational sector within Pakistan. In this policy the education of the nation and Islamic knowledge was particularly focused for reasonable development. Literacy, non-formal, elementary, secondary and teacher education were also included. The government particularly focused vocational and technical education in addition to higher education for the students so that the need of the market can be fulfilled. The government also particularly focused the private sector which is consistently and rapidly developing within Pakistan for providing upto the mark quality education to the nation (http://www. moe. gov. pk). The higher education sector of Pakistan is facing problems due to significant reduction in the budget during the last two years (2010 and 2011). Presently the government is spending around 1. 8 percent of its GDP on education and in the world there are only six countries found which are following Pakistan. The upcoming problems for the education sector of Pakistan are further critical as the government has planned to devolve the higher education system to the provinces till 2014. It will be a huge loss to the upcoming generation as the standard of education will be decreased in addition to huge wastage of money invested for the development of the sector. The policies of the government are negatively effecting the development of education sector although there is crucial requirement of central authority which ensures the development of education sector (newsweekpakistan. com).

Analysis of Peripherin-2 Cone Mutation V268I

Analysis of Peripherin-2 Cone Mutation V268I Introduction: 1.1 Composition of Retina: Eye an organ which is known for its sensory function Vision, serves as a basis for perception of the world outside. Three layers of tissues enclose the eye, outermost layer sclera, middle layer choroid and inner layer retina. Central macular region of the retina called as fovea consist of photoreceptor cells, where maximum visual acuity is possible due to minimal light scattering. Iris found in region between the cornea and lens acts like an aperture controlling the amount of light that could enter and pass through retina (Siegel and Sapru.2011). Retina consists of following layers: The outer most primary epithelium layer with pigmented melanin cells absorbs uncaptured light preventing reflection back to rest of retina. The later parts of retina layers involved in sensing and processing of light stimulus are the layers with photoreceptor cells- light sensitive region with rod and cone cells, external limiting membrane, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer and inner most layer with ganglion cell axon fibers. Optically as light when imaged onto retinal photoreceptor layer, light passing through this layer with rods and cones converts light stimuli to electric signal through horizontal cells, further processed through bipolar, amacrine before they reach ganglion cells. From the axons of Central Nervous System (CNS) ganglion cells information is passed in the form of action potential to optic disc and optic nerve signal is received by brain’s visual center. (Ayoub, 2008). 1.2 Composition of Photoreceptors: Retina is made up of two different types of photo receptors- Rods and cones, the ratio of these photoreceptors vary in different species depending on its habitat being diurnal or nocturnal, in human’s rod cells are in majority with cone cells in a ratio of 20:1 (Mustafi et al., 2009). Rods and cones consist of outer segment, inner segment and synaptic terminal. Towards outer surface retina outer segments are located, these segments contain membranous discs arranged in the form of a stack, and these regions are involved in photo transduction by detecting photons and converting them into electrical signals. Inner segment consist of nucleus, both segments are integrated by a stalk containing microtubules. The synaptic terminal carries out synaptic contact with other cells (Siegel and Sapru. 2011). Retinal pigment epithelial cells phagocytize the aged disks which are shed from distal end when new a new disk is added to the base (Young. 1967). Fig: 1 Structure of rod and cone cell. In rods outer segment disc membrane is separated by plasma membrane, disks consist of enclosing lipid bilayer compartment called as disk lumen or intra distal space. Lamellar region of the disk is joined by curved rim region, lamellar region consist of photoreceptor protein, Rhodopsin – an integral membrane protein, and curved rim regions consist of filaments extending to cytoplasmic regions of adjoining disks maintaining structure of outer segment. Rim regions also contain membrane proteins Rim-1, a high molecular weight protein and two other lower molecular weight proteins Peripherin/rds and Rom-1. In cones discs are not completely formed, they are adjoined and are continuous with plasma membrane. (Molday. 1994). These two proteins Peripherin/rds and Rom-1 are homologous in structure, peripherin/rds is expressed both in rods and cones while Rom-1 is only found in rods, they can interact to form dimers and tetramers. (Elizabeth. 2005) Cones are known to be responsible for vision during daylight and mediate color vision, while rods do not. Axial structure of cones makes them capture light efficiently during day, with fast responsive membrane potential with less integration time. There are three types of cones L, M, S cones which are specific to a region of visible spectrum to which they are found to be sensitive. L cones are sensitive to ÊÅ ½max~ 553-565, M cones are sensitive to ÊÅ ½max~ 530-537, S cones are sensitive to ÊÅ ½max~ 415-430, frequency of photons. (Mustafi et al., 2009). Rods are specialized to work under dim light conditions, rhodopsin is the most abundant protein found in the rods constituting about 85 % of total protein in rod outer segments. (Elizabeth. 2005). Circuit transmitting information to ganglion cells is different in rods and cones, each rod bipolar cell is connected to many rod cells, and many rod bipolar cells are connected by amacrine cell. More convergence make rods better detector of light but reduces spatial resolution. In cones it’s less convergent as each ganglion cell receives input from only one cone bipolar cell, which is connected to single cone cell (Mustafi et al., 2009). Signal transduction in Photoreceptors: The signaling cascade in photoreceptors involves from light absorption till signal generation by membrane polarization, resulting in controlling the rate of release of neurotransmitter through synaptic terminal. In Dark light condition gyanylate cyclases (GCs) actively produce high Cyclic guanosine monophosphate (cGMP) levels, There by the cGMP sensitive cyclic nucleotide-gated (CNG) channel is in its open state and gives rise to the influx of Na+ and Ca+2. Rods and cones are depolarized to ~35 to 45 mV, where outer segment membrane channels are open permeable to Na+ by effluxing k+ balancing cations. This constitutes a circuit known as â€Å"Dark or circulating current†. In this condition with the channel opened for influx of ca+2 initiates the release of neurotransmitter from synaptic terminal. In light conditions cation channel in outer membrane is closed, thereby membrane hyperpolarizes towards equilibrium potential for k+, causing halt in neurotransmitter release (Jindrova. 1998). When photon is absorbed by Rhodopsin- a 40 kDa protein belonging to G protein coupled receptors, consist of a protein opsin, a single polypeptide with seven transmembrane helical segments forming three cytoplasmic loops and a chromophore 11-cis retinal, which binds to lysine, 296 residue on opsin’s seventh helix. In rods and cones photon absorption leads to isomerization of 11-cis retinal form to all trans retinal confirmation and in the surrounding opsin repositioning of cytoplasmic loops occur which in turn activates the protein to Metarhodopsin II (R*) state, which stimulates the heterotrimeric G protein transducin (Hargrave et al.1993; Scot and John . 2008). Preceding from here all the steps occur in dark state, Transducin stimulates cGMP phosphodiesterase (PDE6) to hydrolyze cGMP to 5’GMP by dissociation of ÃŽ ³ subunit from PDE ÃŽ ±ÃŽ ² subunits, resulting a vast increase of 5’GMP and decrease in cGMP concentration leading to the closing of the CNG channel. As a consequence, the outer membrane hyperpolarises to ~-70 mV and release of neurotransmitter is halted to bipolar cells, finally light signal information is sent by termination of transmitter release to the brain. For regeneration of dark current, the Rod cell needs to restore back the concentration of cGMP which is regulated by concentration of Ca+2 and by gyanylate cyclases (GC), Ca+2 molecules are bound by gyanylate activating proteins. In light conditions, where the CNG channels are closed and Ca+2 levels are low, the low level of calcium activates gyanylate activating proteins, which in turn stimulates gyanylate cyclases leading to production of cGMP. Increased level of cGMP causes to open CNG channel and dark current is restored (Jindrova. 1998). Additional mechanisms are found to be involved to restore the molecules from active to inactive state. Inactivation of Rh* by phosphorylation, catalyzed by rhodopsin kinase (Chenet al.1999). Phosphorylated rhodopsin is blocked by the protein arrestin by binding to it preventing activation of transducin, resulting in breakdown of activated rhodopsin (Xu et al,. 1997). The all trans retinal disassociates from opsin, diffuses to cytosol transported to outersegment then into pigmental epithelium where it is reconverted to 11- cis retinal, then the recycled 11 -cis retinal is transported back to outer segments. Peripherin- 2: This membrane protein is named on basis of localization studies done in 1987, which revealed that this protein was found localized around the periphery region of the outer segments, disks in rods and lamellae or disks in cones. (Molday et al,. 1987). In humans peripherin/rds is encoded by cDNA Peripherin/rds with open reading frame of 346 amino acids, weighing 39.3 KDa (Travis et al., 1989). Peripherin is an integral membrane glycoprotein having four trans membrane domains. When photoreceptor cells fail to develop in outer segments it results in retinal disorder named retinal degeneration slow (rds). Amino acid sequence of peripherin in bovine photoreceptor cell is 92.5% identical to protein encoded by rds gene in wild type mouse. Localization of these proteins was also found to be in rod outer segment (ROS) membranes, implying the role of peripherin-2 as a cause in retinal disorder. (Connell et al, .1991). Peripherin protein is also known and addressed with many other names like pho toreceptor peripherin, peripherin-2, peripherin/rds, rds/peripherin and rds. The two dimensional structure proposed consisted of four membrane spanning domains, with its N and C terminals located intracellular in extradiscal space, two extra cellular loops D1 and D2 ( EC1 and EC2) are localized with each containing a N-linked glycan in intradiscal space. The D2 loop among four species mouse, rat, human and bovine is found to be 92% identical, predicted that evolutionary drift in this loop could be a reason for its involvement in protein -protein interactions. (Connell and Molday, 1990; Travis et al., 1991). Fig: 2 Structure of Peripherin-2. D2 loop in intradiskal region is found to be key in protein folding and tetrameric subunit formation, changes in amino acids in this loop resulted in either protein misfolding or reduction in sediment coefficient of protein or even both, seven cysteine residues found in this loop to be important for intramolecular disulphide bonds, one of the cysteine residue is responsible for polymerization of tetramers (Loewen and Molday, 2000). When seven conserved cysteine residues were replaced they showed abnormalities, C214S linked mutant for adRP was not normally folding and interacting with rom-1 protein to form tetramer. While in C150S mutant failed to form intra molecular disulphide bonds, these results revealed that cysteine residues were crucial for folding of protein and subunit assembly, failure of these functions are linked to retinal disorders like adRP. (Goldberg et al., 1997). Human rom-1 protein with 351 amino acids exhibiting 35% identity to peripherin-2, hydropathy profiles revealed that rom-1 and peripherin-2 have a similar topology with four transmembrane domains, one of differences noted in the both the proteins were consensus sequence for N-linked glycosylation is absent in the rom-1 (Molday. 1994). Peripherin homologous protein rom-1 forms disulfide-linked dimers with each other (Bascom et al.,1992). Peripherin-2/rom-1 core native complex was found to exist as a tetramer, membrane curvature was found to induce with tetramer formation when they were reconstructed in lipid vesicles (Kevany et al,. 2013). Peripherin-2 associates with itself and also with its homologue rom-1 in forming homo and heterotetramers core, these tetramers when linked intramolecularly together by disulphide bonding forms octamers and higher order oligomers (Loewen and Molday. 2000). A highly conserved region within the C-terminal domain of peripherin/rds was found to be import ant for membrane fusion, it forms a complex with melanoregulin (MREG) onto the last five residues of the C-terminus (Gln341-Gly346), membrane fusion is important in organelle biogenesis, disc morphogenesis and disc shedding. (Boesze-Battaglia et al., 2007). Peripherin/rds plays a major role in rod and cone outer segment morphogenesis. In mice, absence of peripherin/rds leads to develop normal photoreceptor inner segments but they fail to form outer segments, these outer segments undergo apoptosis (Nir and Papermaster. 1986). Peripherin- 2 is found to have differential roles in rod and cone cells with respect to their binding partners. Peripherin 2 found to be binding with the Glutamic acid rich protein (GARP) portion of ÃŽ ² subunit of rod CNG channel. This interaction is predicted to anchorage disc rim and rod plasma membrane, but in cone CNG channel such interaction was not observed, as GARP is not expressed in cone CNG channel or in other from. (Conley et al,.2010). Peripherin-2 links CNGB1a CNG channel subunit to rhodopsin, these three forms a complex localizing its contact between disk rims and plasma membrane regions. FRET experiments revealed that transmembrane -4 (TM4) of periperin-2 is key for rhodopsin interaction. In peripherin-2, G266D mutation specific to TM4 region, eliminated the specific binding capacity of peripherin-2 with rhodopsin (Becirovic et al,. 2014). Peripherin-2 being a multifunctional protein and mutations in peripherin/RDS gene results in a broad spectrum of retinal disorders like macular dystrophies, cone and cone-rod dystrophies and retinitis pigmentosa. Studying Peripherin/RDS mutations and protein structure elucidates pathophysiological mechanisms underlying these retinal disorders for effective therapeutic intervention (C.J.F. Boon et al,. 2008). Zscan29: At present, very less information is known and available regarding the function, structure and localization of this protein Zscan29. mRNA specific for this protein were found to be expressed in retina through RT- PCR experiments from mice different tissues. In mice 5 different isoforms of this protein were found, with longest isoform having 869 amino acids (aa), and other isoforms were of 834 aa, 548 aa,265 aa,206 aa. The isoform with 265 aa was found to interact with the C- terminus of CNGB1a sub unit of CNG channel. In long isoforms with 869aa and 834aa, a SCAN domain, a DUF2 domain, 2 GT-1 domains and 6 zinc finger domains were found. The scan domain is predicted to be a DNA binding domain and involved in transcription regulation, as this domain in other proteins were also found to be associated as transcription factors. DUF-2 Domain function is unclear and yet to be known, Two GT-1 domains of 79 aa each, were predicted to play a major role in light sensing mechanisms by binding GT trihelix transcription factors. (___citation__). In Arabidopsis GT-1 is predicted to respond to light signals via calcium dependent phosphorylaton, after GT-1 binding to GT cis element of light inducing gene (Bauer et al). Fig: 3 Isoforms of Zscan29 protein with its domains. Aims of this work: Analysis of Peripherin-2 cone mutation V268I: Verification of the interaction of WT peripherin-2 and peripherin-2 with mutation at V268I position with S-opsin, M-opsin and Rhodopsin via co immunoprecipitation from HEK293 cells. Interaction and localization of Peripherin-2, V268I mutation in mouse retina. Analysis of Peripherin-2 mutations at position P210: Verification of the interaction of peripherin-2 protein with mutations at P210 position with wild type Peripherin-2 and its homolog Rom-1 via coimmunoprecipitation from HEK293 cells. In vitro imaging of HEK293 cells expressing peripherin-2 protein with mutation at P210 position. Expression and localization of Peripherin-2, P210L and P210R mutations in mouse retina. Affinity purification of Anti-Zscan29 antibody and checking antibody efficiency.